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KMID : 0613820060160010088
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Journal of Life Science
2006 Volume.16 No. 1 p.88 ~ p.94
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Random Amplified Polymorphic DNA Analysis for Origin Identification of Olive Flounder (Paralichthys olivaceus) and Redlip Croaker (Pseudosciaena polyactis)
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Kang Duk-Jin
Lee Suk-Keun
Jin Deuk-Hee
Choi Suk-Jung
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Abstract
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The random amplified polymorphic DNA (RAPD) technique was investigated as a potential tool for the origin identification of olive flounder (Paralichthys olivaceus) and redlip croaker (Pseudosciaena polyactis). Olive flounder specimens were collected from North Korea and several locations of South Korea (Jumunjin, Tongyoung and Geoje). Fishes obtained from Tongyeong and Geoje were cultured products. Redlip croaker specimens were collected from South Korea and China. Consistent and distinct diagnostic bands were easily identified in the RAPD patterns of the olive flounder specimens. Although consistent diagnostic bands rarely appeared in the RAPD pattern of redlip croaker specimens because of their genetic heterogeneity, we were able to find potential diagnostic bands in the average RAPD pattern of each origin.
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